Pulsed-field gel electrophoresis (PFGE), or clamped homogeneous electrical field electrophoresis (CHEF), is a novel gel electrophoresis type for the separation of DNA macromolecules. This is ...
Investigators often work with complex nucleic acid samples in procedures such as amplification, renaturation, cDNA synthesis and normalization, sonication, digestion, nicking, labeling and ...
Figure 1: Titration of a 214-bp lac promoter DNA fragment with Escherichia coli CAP protein. Footprinting assays require simultaneous optimization of binding by the protein(s) of interest and the ...
Nucleic acid agarose gel electrophoresis is a fundamental technique in molecular biology, boasting a rich history of advancements and innovations spanning over half a century. This technique plays a ...
Pulsed-Field Gel Electrophoresis (PFGE) PFGE was developed in 1984 and has since become the gold standard for bacterial subtyping. PFGE is the primary subtyping method used by PulseNet, a network of ...
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