The Scientist

The History and Evolution of PCR

Explore 40 years of PCR history, from Kary Mullis’s invention story to the rise of digital PCR and how it revolutionized ...
Nature

Degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) and amino linking, a series of amplification reactions used to create representations of each clone (1).

First, the BAC clone DNA is denatured into single stranded templates. Each of the three DOP-PCR primers used in the next step is flanked by known 5' and 3' anchor sequences with six random nucleotides ...