A Panamanian national holiday remembering the killing of 21 protesters by police and U.S. troops in 1964 is taking on new resonance this year as U.S.

Hundreds of Panamanians marched on Thursday to mark the anniversary of a deadly uprising against U.S. control of the Panama Canal in 1964, with some protesters burning an effigy of President-elect Donald Trump who has threatened to retake the vital global waterway.

President-elect Donald J. Trump refused on Tuesday to rule out the use of military or economic coercion to force Panama to give up control of the canal that America built more than a century ago and to push Denmark to sell Greenland to the United States.

Control of the Panama Canal, once a key U.S. presidential campaign issue for Ronald Reagan and an animating topic for people ranging from film legend John Wayne to an unpredictable Canadian-born U.S.

The canal executive said China doesn’t control route and U.S. ships can’t get better rates than others.

The Panama Canal Authority chief dismissed President-elect Trump’s claims that China is controlling the trade route and criticized his comments about fees. Ricaurte Vásquez Morales told The Wall Street Journal that Trump’s accusations that China is running the Panama Canal are “unfounded.

Panama rejected suggestions of Chinese influence over its canal and dismissed threats of possible US military action on January 7, after President-elect Donald Trump refused to rule out force to regain control of the strategic waterway.

The leader of the Panama Canal Authority denied President-elect Donald Trump’s claims that China is controlling the vital trade route and said Trump’s suggestion that U.S. ships get preferential rates “will lead to chaos.